ANTIOXIDANT ACTIVITY
PIGMENT β-CAROTENE BROWN ALGAE
Sargassum cristaefolium FRESH AND “TEA”
Adiwira
Sandrikanata*, Hartati Kartikaningsih, dan Yahya
Teknologi Hasil
Perikanan
Faculty of Fisheries
and Marine Science, Brawijaya University, Malang
*dika.kaka@gmail.com
ABSTRACT
Pigments are substances of natural colours obtained from the
extraction of natural materials, which are derived from plant or animal. The
main pigment found in the tissues of the plant is chlorophyll and karetenoid.
One of the pigments inspected in Sargassum cristaefolium is β-carotene. β-carotene acts as an antioxidant and provitamin A. The function
of antioxidant captures free radicals and prevents the oxidation process in the system that has a
low oxygen pressure. β-carotene
compound has a high vitamin A activity. The purpose of this research was to
know the difference between β-carotene
antioxidant activity in Sargassum cristaefolium “tea” and fresh. Method
research that used on this research was explorative - descriptive method. In
explorative method, β-carotene
identified by TLC (Thin Layer Chromatography), spectrophotometer UV-Vis
analysis, HPLC (High Performance Liquid Chromatography) analysis, L,a,b
analysis with comparing 0hue, antioxidant testing used DPPH method. Experiment
test was used to obtain the difference of antioxidant from Sargassum
cristaefolium fresh and “tea”. The results showed that wavelength
in fresh sample algae was 452,60 nm and had wavelength movement (hipsokromik) 452 nm in samples of
“tea”.
HPLC testing showed that fresh sample algae had retation time 11,276
minutes and sample “tea”
11,827
minutes. The colour of fresh sample algae based on hue degree was 60,8
and sample “
tea” 56,7.
Analysis of DPPH in knowing the content of antioxidant in fresh sample algae
was 124,387 ppm
and sample “
tea” was 132,884 ppm. This research did not found the difference of
antioxidant activity pigments β-carotene in fresh sample algae and “tea”
Key Words : Sargassum
cristaefolium, pigment, beta carotene, fresh,” tea”, and antioxidant
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